Research Associate II - Molecular Cloning

Posted 23 March 2021
SalaryUp to £55000.00 per annum
Job type Permanent
DisciplineLife Science and Pharmaceuticals
Contact NameDeLisa Chambers

Job description

Research Associate II - Molecular Cloning (Direct Hire Opportunity)
The QC/AD Associate II is responsible for supporting manufacturing and testing efforts, reporting into a QC supervisor or manager, with work occasionally directed. The QC/AD Associate II will gain exposure to some of the complex tasks within the job function. Activities include developing and validating methods and conducting testing on raw materials, process intermediates, drug substance, drug product, and stability samples.
Support cloning and cell line development by independently performing: Common PCR- and restriction enzyme-based cloning techniques, E. coli transformations and screens, Plasmid purification, Mammalian cell line transfections, E. coli and mammalian cell line cataloguing and maintenance, Detection, quantification and assessment of cell line and exosomal modifications, using both cell-free (nanoparticle tracking analysis/Nanosight, western blot, ELISA) and in vitro (flow cytometry, microscopy) techniques
  • Develop, plan and execute experiments according to established SOPs and Good Laboratory Practices (GLP) guidelines
  • Maintain detailed records of experimental design, execution and results
  • Troubleshoot and optimize experimental protocols
  • Work collaboratively as part of a broader research team to design and perform experiments to advance corporate strategy
  • Assist in daily laboratory operation and maintenance including equipment checks, maintaining inventory and ordering reagents and supplies
  • Analyze data, write reports and present findings to the team and/or company leadership
  • Assist in in vivo studies, as needed
  • As experimental and other circumstances demand, occasionally work outside of normal business hours

Required Qualifications:
  • Demonstrated ability to independently perform and optimize PCR-based molecular cloning
  • Proficiency in E. coli transformation and screening
  • Proficiency in common western blot techniques
  • Proficiency in endotoxin-free plasmid purification
  • Demonstrated ability to manage multiple tasks/projects efficiently in a fast-paced environment
  • Strong time management, written and verbal communication skills
  • Proven ability to conduct literature searches on PubMed, Google Scholar, etc.
  • Proficiency with standard computer software (including Microsoft products and EndNote or similar) as well as statistical software (SAS, R, JMP, GraphPad Prism or similar)
  • Proven productivity working as a member of a highly collaborative cross-functional team

Preferred Qualifications:
  • Mammalian cell culture including aseptic technique
  • Mammalian cell line transfection
  • Packaging viral constructs and transduction of mammalian cell lines